Crystagen 20 mg vial dosage

What Crystagen is

Class:

Short synthetic peptide sold as a Khavinson-line immune-system bioregulator

Also called:

Immune bioregulator tripeptide

Used for:

Marketed for immune-system support and healthy aging, within the Khavinson peptide-bioregulator line.

Human evidence:

Animal / lab only. PubMed indexes exactly one paper that studies Crystagen by name: a Russian-language report on several short peptides tested together in the ageing spleen. Its abstract names no species and does not say whether the work was done in living animals or in tissue culture. It is not a clinical trial, not a randomised trial, and not a study in a living, dosed person. I could not find any second paper, in any language, that tests Crystagen specifically.

US status:

Research use only. Not approved for human use by the FDA or any other regulator I can verify, and sold as a research chemical.

The full picture, with every study: Crystagen guide.

How to mix a 20 mg Crystagen vial

For this vial: 5 mL of bacteriostatic water makes 4 mg/mL, so 300 mcg is 7.5 units (0.08 mL) on a U-100 insulin syringe. Pick a different volume from the units table if you want bigger or smaller draws; the number of doses stays the same.

1. Wipe the rubber stoppers of both the peptide vial and the bacteriostatic water vial with an alcohol swab and let the alcohol dry. ^[3][4]
2. With a new sterile needle and syringe, draw up the amount of bacteriostatic water you have chosen for the dilution. ^[3][4][5]
3. Inject the water into the peptide vial by aiming the stream against the inside glass wall, which helps prevent foaming. ^[6]
4. Swirl or roll the vial gently until the powder has fully dissolved; do not shake it. Do not inject the solution if it is cloudy or has particles in it. ^[3][4][6]
5. Label the vial with the date and time it was mixed, the amount of water added and how long it can be used after mixing. ^[3]
6. Store the mixed vial in the refrigerator at 2-8°C (36-46°F) and do not freeze it. ^[6]

Step by step with pictures: how to reconstitute peptides. About the water itself: bacteriostatic water.

Storing Crystagen vials

• Freeze-dried (lyophilized) peptide keeps far longer than peptide in solution; peptide suppliers advise storing the powder at -20°C away from bright light. ^[7]
• Protect peptide vials from heat and light. ^[7][8]
• Avoid repeated freeze-thaw cycles; a prescription peptide label also says not to freeze a vial once it has been mixed. ^[6][7]
• Once a multi-dose vial, such as bacteriostatic water, has been opened (needle-punctured), date it and discard it within 28 days unless its manufacturer states another date. ^[9][10]

More detail: peptide storage and shelf life.

Injecting safely

• Clean the injection site with an alcohol swab and let the skin dry completely before injecting. ^[11][12]
• Pinch up a fold of skin and fat (not muscle) and insert the needle at a 45- to 90-degree angle: 90 degrees where there is enough fatty tissue, 45 degrees where there is little. ^[11][12][13]
• Pulling back on the plunger (aspiration) is not necessary for a subcutaneous injection, because the chance of the needle being in a blood vessel is small. ^[11]
• Inject slowly, at roughly 10 seconds per mL. ^[11]
• Common sites are the belly, the thigh and the upper arm; rotate sites, keeping each injection at least 1 inch (2.5 cm) from the last. ^[6][11][12]

Hygiene and warning signs

• Use a new sterile needle and syringe for every injection and every time you enter a vial; never reuse them. ^[5][9][12]
• Never share needles, syringes or pens with anyone, even if the needle is changed; unsafe injection practices spread hepatitis B, hepatitis C and HIV. ^[5][8][9]
• Put used needles and syringes in a sharps disposal container straight away (FDA-cleared if possible), never loose in the trash, recycling or toilet, and dispose of the container following your community's guidelines. ^[14][15]
• Contact a health care provider if the injection site shows signs of infection, such as redness, swelling, red streaks or a fever. ^[12]

Where to inject: subcutaneous injection sites.

How Crystagen works

• Crystagen is one of the short peptides from Vladimir Khavinson's St Petersburg bioregulator programme, which designs short peptides from the amino-acid make-up of tissue extracts on the premise that each acts on its matching tissue. ^[16][17]
• In the one study I found that tests it by name, its reported target was the B-cell arm of the immune system in the spleen, whereas two other peptides in the same study (Vilon and R-1) acted on T-helper cells. ^[17]

What the Crystagen research found

Human evidence: animal / lab only. Each result says what was studied.

• Animal studyA 2014 Russian-language paper from the Khavinson group compared four short peptides - Vilon, Timogen, Crystagen and R-1 - in the ageing spleen and reported that Crystagen activated B-cells. ^[17]
• Animal studyThe same paper reports that, unlike Timogen (which activated B-cells by lowering cell death and raising cell proliferation), Crystagen did not produce cell renewal in the spleen as it aged. ^[17]

Crystagen side effects and risks

I found no published human study of Crystagen, and the one laboratory paper that tests it measured immune-cell activity, not safety, so no side effects are documented.

• Exactly one published study exists for this specific peptide, and it is a laboratory paper on the ageing spleen (its abstract states neither the species nor whether it was done in living animals), not a clinical trial - there is no human data of any kind.
• No dose has research behind it in a living person, so any protocol in circulation was produced by a vendor, not a researcher.
• Sold as a research chemical; contents and purity are unverified by any regulator.

Reported side effects and what the studies measured: Crystagen side effects.

Where this vial size comes from

Crystagen is sold as a 20 mg vial by the pages below, each checked on the date shown. I list them as evidence that this size exists, not as a recommendation to buy from them.

• Next Level PharmvendorLists it as: "Crystagen (20mg)" vial · checked 2026-09-28
• BioEdge Research LabsvendorLists it as: "Crystagen (20mg)"; Fill: 20mg · checked 2026-09-28

The doses come from the sourced common protocol on the Crystagen guide, and every number on this page is arithmetic on those doses and this vial size.

Where to buy Crystagen for research

Sold for research use only. Listed by Next Level Pharm and BioEdge Research Labs; compare them on my vendors page or see the listing on the Crystagen page.

Questions

How much water do I add to a 20 mg Crystagen vial?

There is no single correct amount: water changes how many units a dose is, not how many doses the vial holds. With 5 mL the vial is 4 mg/mL and 300 mcg is 7.5 units (0.08 mL) on a U-100 insulin syringe. The units table gives 5 mL.

How many doses are in a 20 mg Crystagen vial?

100 doses at 200 mcg, 66 doses at 300 mcg, 50 doses at 400 mcg.

How long does a 20 mg Crystagen vial last?

At 300 mcg a dose and once daily during an initial course, about 9.4-16.5 weeks. How long a mixed vial keeps is a separate limit; see storage below.

How many 20 mg vials do I need for a cycle?

1 vial at 200 mcg 4 times a week for 1.4 weeks; 2 vials at 400 mcg 7 times a week for 12 weeks.

What does 1 unit equal in a 20 mg Crystagen vial?

40 mcg with 5 mL of bacteriostatic water, on a U-100 syringe.