How much water do I add to a 10 mg Bronchogen vial?

There is no single correct amount: water changes how many units a dose is, not how many doses the vial holds. With 1 mL the vial is 10 mg/mL and 1.5 mg is 15 units (0.15 mL) on a U-100 insulin syringe. The units table gives 1, 2, 5 mL.

How many doses are in a 10 mg Bronchogen vial?

10 doses at 1 mg, 6 doses at 1.5 mg, 5 doses at 2 mg.

How long does a 10 mg Bronchogen vial last?

At 1.5 mg a dose and once daily, about 0.9 weeks. How long a mixed vial keeps is a separate limit; see storage below.

How many 10 mg vials do I need for a cycle?

1 vial at 1 mg 7 times a week for 1.4 weeks; 7 vials at 2 mg 7 times a week for 4.3 weeks.

What does 1 unit equal in a 10 mg Bronchogen vial?

100 mcg with 1 mL, 50 mcg with 2 mL, 20 mcg with 5 mL of bacteriostatic water, on a U-100 syringe.

Research use only · common protocol

Bronchogen 10 mg vial dosage

A 10 mg Bronchogen vial mixed with 1 mL of bacteriostatic water gives 10 mg/mL, so 1.5 mg is 15 units (0.15 mL) on a U-100 insulin syringe. At 1.5 mg a dose, one vial holds 6 doses.

Email me this plan, with a reminder before the vial's 28-day mark →

The common Bronchogen protocol is 1-2 mg, once daily, consistent time of day - common practice, not a dose established in trials. Evidence, side effects and legal status are on the Bronchogen guide.

Bronchogen 10 mg vial at a glance

Vial: 10 mg of Bronchogen per vial
Common dose: 1-2 mg
How often: once daily, consistent time of day
Mixing example: 1 mL bacteriostatic water = 10 mg/mL; 1 unit = 100 mcg
Doses per vial: 10 at 1 mg · 6 at 1.5 mg · 5 at 2 mg
Cycle: 1.4-4.3 weeks, then 13-26 weeks off

What Bronchogen is

Class:: Synthetic tetrapeptide (Ala-Glu-Asp-Leu)
Also called:: AEDL peptide, Bronchogen tetrapeptide
Used for:: Sold for bronchial and lung support, marketed within the Khavinson peptide-bioregulator line for respiratory and healthy-aging use.
Human evidence:: Animal / lab only. PubMed returns 12 records for "Bronchogen"; five are unrelated case reports about bronchogenic cysts, a coincidental word match, not the peptide. Of the seven real records, none is a clinical trial or a study in a living human being. The most substantive result is real: two related publications report that a month of Bronchogen treatment reduced neutrophilic inflammation and restored bronchial epithelium structure in a rat model of nitrogen-dioxide-induced chronic obstructive pulmonary disease. The rest of the record is in vitro DNA-binding and cell-culture work.
US status:: Research use only. Not approved for human use and sold as a research chemical.

The full picture, with every study: Bronchogen guide.

Units per dose: Bronchogen 10 mg vial

Water added	Concentration	1 unit =	1 mg	1.5 mg	2 mg
1 mL	10 mg/mL	100 mcg	10 units	15 units	20 units
2 mL	5 mg/mL	50 mcg	20 units	30 units	40 units
5 mL	2 mg/mL	20 mcg	50 units	75 units	100 units

U-100 insulin syringe (100 units = 1 mL), to the nearest half unit. Water volumes are examples. Other numbers: dosage calculator.

Every dose from 1 mg to 2 mg, in units

With 1 mL of water in a 10 mg vial (10 mg/mL). Every dose across the common range, so you can read off any dose. The sources give a range, not a week-by-week schedule.

Dose	Draw on a U-100 syringe	Volume	Doses per vial
1 mg	10 units	0.1 mL	10
1.2 mg	12 units	0.12 mL	8
1.4 mg	14 units	0.14 mL	7
1.6 mg	16 units	0.16 mL	6
1.8 mg	18 units	0.18 mL	5
2 mg	20 units	0.2 mL	5

How many doses a 10 mg vial holds

Dose	Doses per vial	Weeks per vial
1 mg	10	1.4
1.5 mg	6	0.9
2 mg	5	0.7

Weeks per vial at the common frequency (once daily, consistent time of day). Doses per vial do not depend on how much water you add.

Email me my Bronchogen 10 mg vial plan

Pick your water volume and dose and I will email you the numbers for this vial - units, volume, doses per vial and how long it lasts - plus the mixing and storage steps, and a reminder before the mixed vial reaches 28 days.

The common Bronchogen protocol

Dose	1-2 mg per injection
How often	once daily, consistent time of day
Timing	Subcutaneous injection into the abdomen or thigh, same time each day.
Route	Subcutaneous injection
Cycle	1.4-4.3 weeks
Break	13-26 weeks
Often paired with	Chonluten
Sources agree?	Partly - the sources differ, and the ranges above cover them

Common practice from the vendors and clinics that publish it, not a dose tested in trials. Where it comes from: [1], [2], [3]. I do not give week-by-week titration: none of these sources states one.

Supplies for one cycle with 10 mg vials

Counted for the low and high ends of the common protocol, mixing each vial with 1 mL of water. One new syringe per injection.

Plan	Injections	10 mg vials	Insulin syringes	Bacteriostatic water	Alcohol swabs
1 mg, 7×/week, 1.4 weeks	10	1	10	1 mL (1 × 10 mL bottle)	22 (1 × 100-pack)
2 mg, 7×/week, 4.3 weeks	31	7	31	7 mL (1 × 10 mL bottle)	76 (1 × 100-pack)

Arithmetic on the protocol and this vial size. Swabs: two per vial mixed (both stoppers) and two per injection (vial stopper and skin). An opened multi-dose vial has a use-by date (see storage), which on a slow schedule can matter more than the dose count. Plan your own numbers with the protocol builder.

How to mix a 10 mg Bronchogen vial

For this vial: 1 mL of bacteriostatic water makes 10 mg/mL, so 1.5 mg is 15 units (0.15 mL) on a U-100 insulin syringe. Pick a different volume from the units table if you want bigger or smaller draws; the number of doses stays the same.

Wipe the rubber stoppers of both the peptide vial and the bacteriostatic water vial with an alcohol swab and let the alcohol dry. ^[4][5]
With a new sterile needle and syringe, draw up the amount of bacteriostatic water you have chosen for the dilution. ^[4][5][6]
Inject the water into the peptide vial by aiming the stream against the inside glass wall, which helps prevent foaming. ^[7]
Swirl or roll the vial gently until the powder has fully dissolved; do not shake it. Do not inject the solution if it is cloudy or has particles in it. ^[4][5][7]
Label the vial with the date and time it was mixed, the amount of water added and how long it can be used after mixing. ^[4]
Store the mixed vial in the refrigerator at 2-8°C (36-46°F) and do not freeze it. ^[7]

Step by step with pictures: how to reconstitute peptides. About the water itself: bacteriostatic water.

Storing Bronchogen vials

Freeze-dried (lyophilized) peptide keeps far longer than peptide in solution; peptide suppliers advise storing the powder at -20°C away from bright light. ^[8]
Protect peptide vials from heat and light. ^[8][9]
Avoid repeated freeze-thaw cycles; a prescription peptide label also says not to freeze a vial once it has been mixed. ^[7][8]
Once a multi-dose vial, such as bacteriostatic water, has been opened (needle-punctured), date it and discard it within 28 days unless its manufacturer states another date. ^[10][11]

More detail: peptide storage and shelf life.

Injecting safely

Clean the injection site with an alcohol swab and let the skin dry completely before injecting. ^[12][13]
Pinch up a fold of skin and fat (not muscle) and insert the needle at a 45- to 90-degree angle: 90 degrees where there is enough fatty tissue, 45 degrees where there is little. ^[12][13][14]
Pulling back on the plunger (aspiration) is not necessary for a subcutaneous injection, because the chance of the needle being in a blood vessel is small. ^[12]
Inject slowly, at roughly 10 seconds per mL. ^[12]
Common sites are the belly, the thigh and the upper arm; rotate sites, keeping each injection at least 1 inch (2.5 cm) from the last. ^[7][12][13]

Hygiene and warning signs

Use a new sterile needle and syringe for every injection and every time you enter a vial; never reuse them. ^[6][10][13]
Never share needles, syringes or pens with anyone, even if the needle is changed; unsafe injection practices spread hepatitis B, hepatitis C and HIV. ^[6][9][10]
Put used needles and syringes in a sharps disposal container straight away (FDA-cleared if possible), never loose in the trash, recycling or toilet, and dispose of the container following your community's guidelines. ^[15][16]
Contact a health care provider if the injection site shows signs of infection, such as redness, swelling, red streaks or a fever. ^[13]

Where to inject: subcutaneous injection sites.

How Bronchogen works

Bronchogen is a synthetic four-amino-acid peptide from the Khavinson bioregulator programme. Two of the developers' papers (2011 and 2012) give it as Ala-Glu-Asp-Leu, but their 2011 DNA-melting study prints it as Ala-Asp-Glu-Leu. ^[17][18][19]
Test-tube studies report that it binds DNA: it raised the melting temperature of DNA by 3.1°C, binding both strands mainly at the bases, and in fluorescence tests it bound preferentially to sequences containing CTG. ^[18][19]
In cultured human embryonic bronchial epithelial cells, bronchogen increased expression of cell-differentiation factors, with a stronger effect in aged (late-passage) cultures than in young ones. ^[17]

What the Bronchogen research found

Human evidence: animal / lab only. Each result says what was studied.

Animal studyIn rats given chronic obstructive pulmonary disease by 60 days of intermittent nitrogen dioxide exposure, a month of bronchogen reversed airway remodelling (goblet-cell overgrowth, squamous metaplasia, emphysema), restored ciliated cells, raised secretory IgA and reduced neutrophilic inflammation. ^[20]
Animal studyA 2017 report from the same group, using the same rat model, found bronchogen normalised inflammatory cells, cytokines and enzymes in lung fluid and raised secretory IgA and surfactant protein B. ^[21]
Lab studyIn human embryonic bronchial epithelial cell cultures, the tetrapeptide Ala-Asp-Glu-Leu (the sequence printed for bronchogen in the 2011 DNA-melting study) raised proliferation markers most strongly in 'old' late-passage cultures and changed the activity of genes for bronchial differentiation, mucins and surfactant. ^[19][22]
Animal studyIn organotypic cultures of tissue from young (3-week-old) and aged (18-month-old) rats, bronchogen at 0.05 ng/ml had a stimulating effect on lung explants compared with untreated explants; it was one of four peptides each tested on its matching tissue. ^[23]

Bronchogen side effects and risks

I found no published human study of bronchogen, and the rat and cell studies I read measured lung structure, inflammation and gene activity, not safety or tolerability.

No human trial of any kind has been published for Bronchogen, at any dose, for any use.
The only disease-model efficacy data is in rats with chemically induced COPD, not in people.
Sold as a research chemical, so the contents, purity and even the peptide identity of a given vial are unverified by any regulator.

Reported side effects and what the studies measured: Bronchogen side effects.

Where this vial size comes from

Bronchogen is sold as a 10 mg vial by the page below, each checked on the date shown. I list them as evidence that this size exists, not as a recommendation to buy from them.

MA Research ChemsvendorLists it as: 10 mg per vial · checked 2026-09-28

The doses come from the sourced common protocol on the Bronchogen guide, and every number on this page is arithmetic on those doses and this vial size.

Other Bronchogen vial sizes

10 mg
20 mg vial

Where to buy Bronchogen for research

Sold for research use only. Listed by BioEdge Research Labs; compare them on my vendors page or see the listing on the Bronchogen page.

Questions

How much water do I add to a 10 mg Bronchogen vial?: There is no single correct amount: water changes how many units a dose is, not how many doses the vial holds. With 1 mL the vial is 10 mg/mL and 1.5 mg is 15 units (0.15 mL) on a U-100 insulin syringe. The units table gives 1, 2, 5 mL.
How many doses are in a 10 mg Bronchogen vial?: 10 doses at 1 mg, 6 doses at 1.5 mg, 5 doses at 2 mg.
How long does a 10 mg Bronchogen vial last?: At 1.5 mg a dose and once daily, about 0.9 weeks. How long a mixed vial keeps is a separate limit; see storage below.
How many 10 mg vials do I need for a cycle?: 1 vial at 1 mg 7 times a week for 1.4 weeks; 7 vials at 2 mg 7 times a week for 4.3 weeks.
What does 1 unit equal in a 10 mg Bronchogen vial?: 100 mcg with 1 mL, 50 mcg with 2 mL, 20 mcg with 5 mL of bacteriostatic water, on a U-100 syringe.

References (23 sources)

[1] Peptide Mind: 1.5 mg SC once daily, focused ~2-week injectable course, repeated a few times a year rather than continuously, 10/20mg vials reconstituted with 2mL BAC water (vendor, checked 2026-09-28)
[2] Peptides.wiki: Standard course 1mg daily SC (range 1-2mg, some report 500mcg) or 2 capsules daily, 10-30 days, break of several months (other, checked 2026-09-28)
[3] Peptide Dosing Hub: 1.0mg standard / 2.0mg advanced, SC once daily, 10-20 day course, repeated every 4-6 months (other, checked 2026-09-28)
[4] OpenStax, Rice University: 12.3 Preparing Unit-Dose Packaged Medications - Clinical Nursing Skills
[5] East Carolina University College of Nursing: Reconstitution of Medication
[6] Centers for Disease Control and Prevention: Considerations for Blood Glucose Monitoring and Insulin Administration
[7] DailyMed, National Library of Medicine: SEROSTIM (somatropin) for injection - prescribing information and instructions for use
[8] GenScript: Peptide Storage and Handling Guidelines
[9] DailyMed, National Library of Medicine: OZEMPIC (semaglutide) injection - prescribing information
[10] Centers for Disease Control and Prevention: Preventing Unsafe Injection Practices
[11] Pfizer: Bacteriostatic Water for Injection, USP - prescribing information
[12] OpenStax, Rice University: 12.5 Administering Subcutaneous Injections - Clinical Nursing Skills
[13] MedlinePlus, National Library of Medicine: Subcutaneous (SQ) injections
[14] Centers for Disease Control and Prevention: Chapter 6: Vaccine Administration - Pink Book
[15] US Food and Drug Administration: Best Way to Get Rid of Used Needles and Other Sharps
[16] US Food and Drug Administration: DOs and DON'Ts of Proper Sharps Disposal
[17] Khavinson VKh, Linkova NS, Polyakova VO et al. Peptides tissue-specifically stimulate cell differentiation during their aging. Bull Exp Biol Med. 2012;153(1):148-51.
[18] Fedoreyeva LI, Kireev II, Khavinson VKh et al. Penetration of short fluorescence-labeled peptides into the nucleus in HeLa cells and in vitro specific interaction of the peptides with deoxyribooligonucleotides and DNA. Biochemistry (Mosc). 2011;76(11):1210-9.
[19] Monaselidze JR, Khavinson VKh, Gorgoshidze MZ et al. Effect of the peptide bronchogen (Ala-Asp-Glu-Leu) on DNA thermostability. Bull Exp Biol Med. 2011;150(3):375-7.
[20] Kuzubova NA, Lebedeva ES, Dvorakovskaya IV et al. Modulating Effect of Peptide Therapy on the Morphofunctional State of Bronchial Epithelium in Rats with Obstructive Lung Pathology. Bull Exp Biol Med. 2015;159(5):685-8.
[21] Titova ON, Kuzubova NA, Lebedeva ES et al. [ANTIINFLAMMATORY AND REGENERATIVE EFFECT OF PEPTIDE THERAPY IN THE MODEL OF OBSTRUCTIVE LUNG PATHOLOGY]. Ross Fiziol Zh Im I M Sechenova. 2017;103(2):201-8.
[22] Khavinson VKh, Tendler SM, Vanyushin BF et al. Peptide regulation of gene expression and protein synthesis in bronchial epithelium. Lung. 2014;192(5):781-91.
[23] Zakutskiĭ AN, Chalisova NI, Ryzhak GA et al. [The tissue-specific effect of synthetic peptides-biologic regulators in organotypic tissues culture in young and old rats]. Adv Gerontol. 2006;19:93-6.