NAD+ (injection) 1,000 mg vial dosage

What NAD+ (injection) is

Class:

Dinucleotide coenzyme - not a peptide

Also called:

NAD+, Nicotinamide Adenine Dinucleotide, NAD Injection, NAD IV Therapy

Used for:

Sold by IV-therapy clinics and compounding pharmacies, by subcutaneous injection or IV infusion, for marketed energy, anti-aging, cognition and addiction-recovery claims.

Human evidence:

Limited human data. Two published human studies of IV NAD+ are in my verified registry: a 2019 pharmacokinetic pilot tracking plasma and urine NAD+ metabolites over a 6-hour infusion, and a 2026 retrospective clinic-record comparison of IV NAD+ against IV nicotinamide riboside measuring tolerability and short-term safety markers. Both are pharmacokinetic or tolerability studies, not controlled efficacy trials - neither measured the energy, anti-aging, cognitive or addiction-recovery outcomes marketed to consumers, so those claims remain unverified against my registry.

US status:

Unapproved drug. No injectable or IV NAD+ product is FDA-approved for any indication. It reaches consumers through 503A/503B compounding pharmacies and IV-therapy clinics rather than through FDA drug review. FDA's warning letter to compounder GenoGenix LLC, dated 20 January 2026, calls its NAD+ product an unapproved new drug and documents that three patients developed low blood pressure, uncontrollable shaking, shivers and body aches after infusion from the same vial lot, an unopened vial of which tested positive for "excessive bacterial endotoxins with a reported value of 3,360 EU/mL."

The full picture, with every study: NAD+ (injection) guide.

How to mix a 1,000 mg NAD+ (injection) vial

For this vial: 10 mL of bacteriostatic water makes 100 mg/mL, so 275 mg is 2.75 mL (more than one 1 mL syringe holds). Pick a different volume from the units table if you want bigger or smaller draws; the number of doses stays the same.

1. Wipe the rubber stoppers of both the peptide vial and the bacteriostatic water vial with an alcohol swab and let the alcohol dry. ^[3][4]
2. With a new sterile needle and syringe, draw up the amount of bacteriostatic water you have chosen for the dilution. ^[3][4][5]
3. Inject the water into the peptide vial by aiming the stream against the inside glass wall, which helps prevent foaming. ^[6]
4. Swirl or roll the vial gently until the powder has fully dissolved; do not shake it. Do not inject the solution if it is cloudy or has particles in it. ^[3][4][6]
5. Label the vial with the date and time it was mixed, the amount of water added and how long it can be used after mixing. ^[3]
6. Store the mixed vial in the refrigerator at 2-8°C (36-46°F) and do not freeze it. ^[6]

Step by step with pictures: how to reconstitute peptides. About the water itself: bacteriostatic water.

Storing NAD+ (injection) vials

• Freeze-dried (lyophilized) peptide keeps far longer than peptide in solution; peptide suppliers advise storing the powder at -20°C away from bright light. ^[7]
• Protect peptide vials from heat and light. ^[7][8]
• Avoid repeated freeze-thaw cycles; a prescription peptide label also says not to freeze a vial once it has been mixed. ^[6][7]
• Once a multi-dose vial, such as bacteriostatic water, has been opened (needle-punctured), date it and discard it within 28 days unless its manufacturer states another date. ^[9][10]

More detail: peptide storage and shelf life.

Injecting safely

• Clean the injection site with an alcohol swab and let the skin dry completely before injecting. ^[11][12]
• Pinch up a fold of skin and fat (not muscle) and insert the needle at a 45- to 90-degree angle: 90 degrees where there is enough fatty tissue, 45 degrees where there is little. ^[11][12][13]
• Pulling back on the plunger (aspiration) is not necessary for a subcutaneous injection, because the chance of the needle being in a blood vessel is small. ^[11]
• Inject slowly, at roughly 10 seconds per mL. ^[11]
• Common sites are the belly, the thigh and the upper arm; rotate sites, keeping each injection at least 1 inch (2.5 cm) from the last. ^[6][11][12]

Hygiene and warning signs

• Use a new sterile needle and syringe for every injection and every time you enter a vial; never reuse them. ^[5][9][12]
• Never share needles, syringes or pens with anyone, even if the needle is changed; unsafe injection practices spread hepatitis B, hepatitis C and HIV. ^[5][8][9]
• Put used needles and syringes in a sharps disposal container straight away (FDA-cleared if possible), never loose in the trash, recycling or toilet, and dispose of the container following your community's guidelines. ^[14][15]
• Contact a health care provider if the injection site shows signs of infection, such as redness, swelling, red streaks or a fever. ^[12]

Where to inject: subcutaneous injection sites.

How NAD+ (injection) works

• NAD+ (nicotinamide adenine dinucleotide) is a vitamin B3-derived cofactor, not a peptide. Cells use it to carry electrons in redox reactions, which makes it central to energy metabolism. ^[16][17]
• It is also used up by enzymes that do not run redox reactions, including sirtuins, CD38 and PARPs, which link it to DNA repair, chromatin remodelling, cellular senescence and immune-cell function. ^[17]
• Tissue NAD+ levels fall with age in rodents and humans, which is the rationale for trying to raise them. Whether raising NAD+ is safe or beneficial in ageing humans has not been established. ^[17]
• In a small pilot study, NAD+ infused into a vein was removed from the plasma quickly and completely for at least the first 2 hours. The breakdown products seen fit with enzymes that split NAD+ apart (NAD+ glycohydrolase and pyrophosphatase). ^[18]

What the NAD+ (injection) research found

Human evidence: limited human data. Each result says what was studied.

• Human studyIn a 2019 pilot study of a 6-hour intravenous NAD+ infusion, plasma NAD+ and its breakdown products did not change for the first 2 hours. By 6 hours, NAD+ and methylnicotinamide were rising in the urine. ^[18]
• Human studyIn a 2026 retrospective review of clinic records, where clients had 500 mg of NAD+ or nicotinamide riboside by IV on four consecutive days, neither group showed significant changes in the liver enzymes ALT and AST, hsCRP, kidney markers (BUN/creatinine) or TSH over 30 days. In an exploratory analysis, HDL cholesterol fell in the NAD+ group. ^[19]
• Human studyA 2026 systematic review of 113 studies (33 in humans) found no outcome trials of intravenous or intramuscular NAD+ for anti-ageing or wellness. Oral precursors (NR, NMN) reliably raised NAD+ markers, but their effects on functional and health outcomes were mixed and often null. ^[20]
• Human studyFor comparison with injection, a 2026 randomised study in 65 healthy adults found that 14 days of oral nicotinamide riboside or NMN raised circulating NAD+, while nicotinamide did not. ^[16]

NAD+ (injection) side effects and risks

No controlled trial has published systematic safety data for repeated NAD+ injection or infusion; the human safety data cited here are one retrospective clinic-record review, one pharmacokinetic pilot and one FDA enforcement letter.

• Human studyIn the 2026 clinic-record review, people given IV NAD+ reported moderate to severe gastrointestinal symptoms, a faster heart rate and chest pressure during infusion. These were more pronounced than with IV nicotinamide riboside and resolved once the infusion finished. ^[19]
• Human studyBecause of those symptoms, NAD+ infusions took longer to give: an average of 97 minutes, against 37 minutes for nicotinamide riboside. ^[19]
• Regulator reviewAn FDA warning letter describes three patients who developed low blood pressure, uncontrollable shaking, shivers and body aches during or shortly after being given a compounded NAD+ product from the same vial, and were sent to the emergency room. The firm had not tested the finished product before release, and an unopened vial from that lot contained excessive bacterial endotoxins (3,360 EU/mL), so this reflects contamination rather than a known effect of NAD+. ^[21]

Other things to know

• No injectable or IV NAD+ product is FDA-approved, so identity, sterility and potency are not independently verified the way an FDA-approved drug's are.
• The one comparative human study found IV NAD+ caused moderate-to-severe gastrointestinal symptoms, increased heart rate and chest pressure - more pronounced than IV nicotinamide riboside in the same clinic records, needing longer infusion times (about 97 minutes vs 37) to manage.
• FDA has taken enforcement action against at least one NAD+ compounder after three patients needed emergency treatment and a tested vial lot showed excessive bacterial endotoxins.
• No long-term human safety data exist for repeated NAD+ injection or infusion at the doses clinics typically use.

Reported side effects and what the studies measured: NAD+ (injection) side effects.

Where this vial size comes from

NAD+ (injection) is sold as a 1,000 mg vial by the pages below, each checked on the date shown. I list them as evidence that this size exists, not as a recommendation to buy from them.

• Next Level PharmvendorLists it as: NAD+ size options: 500mg, 1000mg · checked 2026-09-28
• Futureproof PeptidesvendorLists it as: "NAD+ 1000mg Vial"; Content: 1000mg per vial · checked 2026-09-28

The doses come from the sourced common protocol on the NAD+ (injection) dosing page, and every number on this page is arithmetic on those doses and this vial size.

Other NAD+ (injection) vial sizes

• 500 mg vial
• 750 mg vial
• 1,000 mg

Where to buy NAD+ (injection) for research

Sold for research use only. Listed by Next Level Pharm, Spartan Peptides and BioEdge Research Labs; compare them on my vendors page or see the listing on the NAD+ (injection) page.

Questions

How much water do I add to a 1,000 mg NAD+ (injection) vial?

There is no single correct amount: water changes how many units a dose is, not how many doses the vial holds. With 10 mL the vial is 100 mg/mL and 275 mg is 2.75 mL (more than one 1 mL syringe holds). The units table gives 5, 10 mL.

How many doses are in a 1,000 mg NAD+ (injection) vial?

20 doses at 50 mg, 3 doses at 275 mg, 2 doses at 500 mg.

How long does a 1,000 mg NAD+ (injection) vial last?

At 275 mg a dose and 1-3 times a week by subcutaneous injection, about 1-3 weeks. How long a mixed vial keeps is a separate limit; see storage below.

How many 1,000 mg vials do I need for a 4-week run?

1 vial at 50 mg 1 time a week for 4 weeks; 6 vials at 500 mg 3 times a week for 4 weeks.

What does 1 unit equal in a 1,000 mg NAD+ (injection) vial?

2 mg with 5 mL, 1 mg with 10 mL of bacteriostatic water, on a U-100 syringe.