How much water do I add to a 10 mg DSIP vial?

There is no single correct amount: water changes how many units a dose is, not how many doses the vial holds. With 2 mL the vial is 5 mg/mL and 300 mcg is 6 units (0.06 mL) on a U-100 insulin syringe. The units table gives 2, 3, 5 mL.

How many doses are in a 10 mg DSIP vial?

100 doses at 100 mcg, 33 doses at 300 mcg, 20 doses at 500 mcg.

How long does a 10 mg DSIP vial last?

At 300 mcg a dose and once nightly, about 4.7-33 weeks. How long a mixed vial keeps is a separate limit; see storage below.

How many 10 mg vials do I need for a cycle?

1 vial at 100 mcg 1 time a week for 4 weeks; 5 vials at 500 mcg 7 times a week for 12 weeks.

What does 1 unit equal in a 10 mg DSIP vial?

50 mcg with 2 mL, 33.33 mcg with 3 mL, 20 mcg with 5 mL of bacteriostatic water, on a U-100 syringe.

Research use only · common protocol

DSIP 10 mg vial dosage

A 10 mg DSIP vial mixed with 2 mL of bacteriostatic water gives 5 mg/mL, so 300 mcg is 6 units (0.06 mL) on a U-100 insulin syringe. At 300 mcg a dose, one vial holds 33 doses.

Email me this plan, with a reminder before the vial's 28-day mark →

The common DSIP protocol is 100-500 mcg, once nightly, 30-60 minutes before intended sleep onset; anywhere from 5-14 nights a month to nightly use depending on the protocol - common practice, not a dose established in trials. Evidence, side effects and legal status are on the full DSIP dosing page.

DSIP 10 mg vial at a glance

Vial: 10 mg of DSIP per vial
Common dose: 100-500 mcg
How often: once nightly, 30-60 minutes before intended sleep onset; anywhere from 5-14 nights a month to nightly use depending on the protocol
Mixing example: 2 mL bacteriostatic water = 5 mg/mL; 1 unit = 50 mcg
Doses per vial: 100 at 100 mcg · 33 at 300 mcg · 20 at 500 mcg
Cycle: 4-12 weeks

What DSIP is

Class:: Nonapeptide
Also called:: Delta sleep-inducing peptide, Emideltide
Used for:: Sold for sleep. Named in the 1970s after a proposed role in promoting delta-wave sleep.
Human evidence:: Limited human data. Six records tagged as randomised trials across a literature of 519 papers, and only one concerns the marketed use - DSIP in chronic insomniacs, published in 1992. The others cover endocrine measures, a glucocorticoid and REM sleep study where DSIP sits in the design, and anaesthesia depth monitoring.
US status:: Research use only. Not approved for human use and sold as a research chemical, where the contents of the vial are not verified.

The full picture, with every study: DSIP guide.

Units per dose: DSIP 10 mg vial

Water added	Concentration	1 unit =	100 mcg	300 mcg	500 mcg
2 mL	5 mg/mL	50 mcg	2 units	6 units	10 units
3 mL	3.33 mg/mL	33.33 mcg	3 units	9 units	15 units
5 mL	2 mg/mL	20 mcg	5 units	15 units	25 units

U-100 insulin syringe (100 units = 1 mL), to the nearest half unit. Water volumes are examples. Other numbers: dosage calculator.

Every dose from 100 mcg to 500 mcg, in units

With 2 mL of water in a 10 mg vial (5 mg/mL). Every dose across the common range, so you can read off any dose. The sources give a range, not a week-by-week schedule.

Dose	Draw on a U-100 syringe	Volume	Doses per vial
100 mcg	2 units	0.02 mL	100
150 mcg	3 units	0.03 mL	66
200 mcg	4 units	0.04 mL	50
250 mcg	5 units	0.05 mL	40
300 mcg	6 units	0.06 mL	33
350 mcg	7 units	0.07 mL	28
400 mcg	8 units	0.08 mL	25
450 mcg	9 units	0.09 mL	22
500 mcg	10 units	0.1 mL	20

How many doses a 10 mg vial holds

Dose	Doses per vial	Weeks per vial
100 mcg	100	14.3-100
300 mcg	33	4.7-33
500 mcg	20	2.9-20

Weeks per vial at the common frequency (once nightly, 30-60 minutes before intended sleep onset; anywhere from 5-14 nights a month to nightly use depending on the protocol). Doses per vial do not depend on how much water you add.

Email me my DSIP 10 mg vial plan

Pick your water volume and dose and I will email you the numbers for this vial - units, volume, doses per vial and how long it lasts - plus the mixing and storage steps, and a reminder before the mixed vial reaches 28 days.

The common DSIP protocol

Dose	100-500 mcg per injection
How often	once nightly, 30-60 minutes before intended sleep onset; anywhere from 5-14 nights a month to nightly use depending on the protocol
Timing	30-60 minutes before intended sleep onset. A named clinic describes individualized 4-8 week initial cycles, sometimes extended to 12+ weeks, rather than a single fixed cycle length.
Route	Subcutaneous injection
Cycle	4-12 weeks
Break	No break length is stated consistently by the sources
Often paired with	Ipamorelin, Selank, CJC-1295
Sources agree?	Partly - the sources differ, and the ranges above cover them

Common practice from the vendors and clinics that publish it, not a dose tested in trials. Where it comes from: [1], [2], [3]. I do not give week-by-week titration: none of these sources states one.

Supplies for one cycle with 10 mg vials

Counted for the low and high ends of the common protocol, mixing each vial with 2 mL of water. One new syringe per injection.

Plan	Injections	10 mg vials	Insulin syringes	Bacteriostatic water	Alcohol swabs
100 mcg, 1×/week, 4 weeks	4	1	4	2 mL (1 × 10 mL bottle)	10 (1 × 100-pack)
500 mcg, 7×/week, 12 weeks	84	5	84	10 mL (1 × 10 mL bottle)	178 (2 × 100-pack)

Arithmetic on the protocol and this vial size. Swabs: two per vial mixed (both stoppers) and two per injection (vial stopper and skin). An opened multi-dose vial has a use-by date (see storage), which on a slow schedule can matter more than the dose count. Plan your own numbers with the protocol builder.

How to mix a 10 mg DSIP vial

For this vial: 2 mL of bacteriostatic water makes 5 mg/mL, so 300 mcg is 6 units (0.06 mL) on a U-100 insulin syringe. Pick a different volume from the units table if you want bigger or smaller draws; the number of doses stays the same.

Wipe the rubber stoppers of both the peptide vial and the bacteriostatic water vial with an alcohol swab and let the alcohol dry. ^[4][5]
With a new sterile needle and syringe, draw up the amount of bacteriostatic water you have chosen for the dilution. ^[4][5][6]
Inject the water into the peptide vial by aiming the stream against the inside glass wall, which helps prevent foaming. ^[7]
Swirl or roll the vial gently until the powder has fully dissolved; do not shake it. Do not inject the solution if it is cloudy or has particles in it. ^[4][5][7]
Label the vial with the date and time it was mixed, the amount of water added and how long it can be used after mixing. ^[4]
Store the mixed vial in the refrigerator at 2-8°C (36-46°F) and do not freeze it. ^[7]

Step by step with pictures: how to reconstitute peptides. About the water itself: bacteriostatic water.

Storing DSIP vials

Freeze-dried (lyophilized) peptide keeps far longer than peptide in solution; peptide suppliers advise storing the powder at -20°C away from bright light. ^[8]
Protect peptide vials from heat and light. ^[8][9]
Avoid repeated freeze-thaw cycles; a prescription peptide label also says not to freeze a vial once it has been mixed. ^[7][8]
Once a multi-dose vial, such as bacteriostatic water, has been opened (needle-punctured), date it and discard it within 28 days unless its manufacturer states another date. ^[10][11]

More detail: peptide storage and shelf life.

Injecting safely

Clean the injection site with an alcohol swab and let the skin dry completely before injecting. ^[12][13]
Pinch up a fold of skin and fat (not muscle) and insert the needle at a 45- to 90-degree angle: 90 degrees where there is enough fatty tissue, 45 degrees where there is little. ^[12][13][14]
Pulling back on the plunger (aspiration) is not necessary for a subcutaneous injection, because the chance of the needle being in a blood vessel is small. ^[12]
Inject slowly, at roughly 10 seconds per mL. ^[12]
Common sites are the belly, the thigh and the upper arm; rotate sites, keeping each injection at least 1 inch (2.5 cm) from the last. ^[7][12][13]

Hygiene and warning signs

Use a new sterile needle and syringe for every injection and every time you enter a vial; never reuse them. ^[6][10][13]
Never share needles, syringes or pens with anyone, even if the needle is changed; unsafe injection practices spread hepatitis B, hepatitis C and HIV. ^[6][9][10]
Put used needles and syringes in a sharps disposal container straight away (FDA-cleared if possible), never loose in the trash, recycling or toilet, and dispose of the container following your community's guidelines. ^[15][16]
Contact a health care provider if the injection site shows signs of infection, such as redness, swelling, red streaks or a fever. ^[13]

Where to inject: subcutaneous injection sites.

How DSIP works

DSIP is a nine-amino-acid peptide, Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu, isolated in the 1970s from the cerebral venous blood of rabbits given sleep-inducing electrical stimulation of the thalamus. ^[17][18]
It was named for its effect in rabbits: infused into the brain, synthetic DSIP raised slow-wave (delta) EEG activity by an average of 35% compared with control animals, while related fragments and analogues did not. ^[18]
A 2006 review noted that no gene, precursor protein or receptor for DSIP had been isolated, and called the case for DSIP as a sleep factor extremely poorly documented, noting that in the authors' own rabbit and rat work some synthetic analogues promoted slow-wave sleep but DSIP itself did not. ^[19]
DSIP is broken down quickly in blood: incubated in human or rat blood it releases breakdown products that behave like tryptophan on gel filtration, and the authors attributed the rapid disappearance of injected DSIP to this degradation. ^[20]

What the DSIP research found

Human evidence: limited human data. Each result says what was studied.

Human studyIn a 1992 double-blind study of 16 chronic insomniacs given three intravenous doses over a five-night laboratory stay, DSIP improved measured sleep efficiency and shortened the time to fall asleep versus placebo, but the authors judged these effects weak, subjective sleep quality did not change, and they concluded it was unlikely to be of major therapeutic benefit. ^[21]
Human studyA 1983 summary of five double-blind intravenous studies by DSIP's discoverers reported that four consecutive injections normalised disturbed sleep in insomniacs and that DSIP raised daytime alertness. The abstract gives no participant numbers. ^[22]
Human studyIn a 2009 randomised study of 24 women having general anaesthesia (12 given DSIP, 12 saline), intravenous DSIP did the opposite of what its name suggests: it reduced EEG delta rhythm, and the authors concluded it probably lightened measured anaesthetic depth. ^[23]
Human studyStudies in healthy men disagree on the stress hormone ACTH. Single intravenous doses lowered plasma ACTH in 11 men in 1989 and in 8 men in 1994, but a 1995 study found DSIP did not change ACTH or cortisol responses to CRH or to a meal. ^[24][25][26]
Human studyIn eight healthy women, intravenous DSIP did not change growth hormone or prolactin secretion, whether measured at rest, overnight or after stimulation with arginine. ^[27]

DSIP side effects and risks

None of the human studies I found published systematic safety data for DSIP, and all of them gave it intravenously under supervision rather than by repeated subcutaneous injection.

Human studyIn the 2009 anaesthesia study, intravenous DSIP significantly increased heart rate and reduced heart rate variability, which the authors said probably reflected reduced parasympathetic tone. ^[23]
Human studyThe 1983 report on five intravenous studies described tolerability as good and said slow injection proved essential, but gave no adverse-event counts. ^[22]
Human studyThe 1992 insomnia trial and the 1994 hormone study report sleep and hormone outcomes only; neither abstract (the 1994 one is truncated on PubMed) mentions adverse events. ^[21][25]

Other things to know

The compound name states a hypothesis from the 1970s and reads as a finding, which is most of its persuasive power in marketing.
Publication peaked at 233 papers in 1985-1994 and has fallen to 11 since 2015. The evidence base is not growing and is unlikely to answer the question soon.
The only randomised trial in the marketed population is from 1992, so no modern protocol has research behind it.

Reported side effects and what the studies measured: DSIP side effects.

Where this vial size comes from

DSIP is sold as a 10 mg vial by the pages below, each checked on the date shown. I list them as evidence that this size exists, not as a recommendation to buy from them.

Next Level PharmvendorLists it as: Size dropdown options: 2mg / 5mg / 10mg · checked 2026-09-28
BioEdge Research LabsvendorLists it as: Size dropdown options: 5mg / 10mg · checked 2026-09-28

The doses come from the sourced common protocol on the DSIP dosing page, and every number on this page is arithmetic on those doses and this vial size.

Other DSIP vial sizes

5 mg vial
10 mg

Where to buy DSIP for research

Sold for research use only. Listed by Next Level Pharm and BioEdge Research Labs; compare them on my vendors page or see the listing on the DSIP page.

Questions

How much water do I add to a 10 mg DSIP vial?: There is no single correct amount: water changes how many units a dose is, not how many doses the vial holds. With 2 mL the vial is 5 mg/mL and 300 mcg is 6 units (0.06 mL) on a U-100 insulin syringe. The units table gives 2, 3, 5 mL.
How many doses are in a 10 mg DSIP vial?: 100 doses at 100 mcg, 33 doses at 300 mcg, 20 doses at 500 mcg.
How long does a 10 mg DSIP vial last?: At 300 mcg a dose and once nightly, about 4.7-33 weeks. How long a mixed vial keeps is a separate limit; see storage below.
How many 10 mg vials do I need for a cycle?: 1 vial at 100 mcg 1 time a week for 4 weeks; 5 vials at 500 mcg 7 times a week for 12 weeks.
What does 1 unit equal in a 10 mg DSIP vial?: 50 mcg with 2 mL, 33.33 mcg with 3 mL, 20 mcg with 5 mL of bacteriostatic water, on a U-100 syringe.

References (28 sources)

[1] PeptideDosage.org: 100-500 mcg SC once nightly 30-60 min before sleep, 5-14 nights/month rather than continuous, 5mg (also 10mg) vial; sometimes paired with ipamorelin or CJC-1295 (vendor, checked 2026-09-27)
[2] Beverly Hills Rejuvenation Center: 2mg dose size offered; typically dosed evening, 30-60 minutes before desired sleep time; individualized protocols with 4-8 week initial cycles, sometimes extended to 12+ weeks; dosing frequency ranges from nightly to several times per week depending on sleep needs (clinic, checked 2026-09-27)
[3] Beverly Hills Rejuvenation Center: Sells a Semax/Selank/DSIP combination product with DSIP dosed at 2mg per dose in the blend, confirming Selank as a real-world pairing for DSIP (clinic, checked 2026-09-27)
[4] OpenStax, Rice University: 12.3 Preparing Unit-Dose Packaged Medications - Clinical Nursing Skills
[5] East Carolina University College of Nursing: Reconstitution of Medication
[6] Centers for Disease Control and Prevention: Considerations for Blood Glucose Monitoring and Insulin Administration
[7] DailyMed, National Library of Medicine: SEROSTIM (somatropin) for injection - prescribing information and instructions for use
[8] GenScript: Peptide Storage and Handling Guidelines
[9] DailyMed, National Library of Medicine: OZEMPIC (semaglutide) injection - prescribing information
[10] Centers for Disease Control and Prevention: Preventing Unsafe Injection Practices
[11] Pfizer: Bacteriostatic Water for Injection, USP - prescribing information
[12] OpenStax, Rice University: 12.5 Administering Subcutaneous Injections - Clinical Nursing Skills
[13] MedlinePlus, National Library of Medicine: Subcutaneous (SQ) injections
[14] Centers for Disease Control and Prevention: Chapter 6: Vaccine Administration - Pink Book
[15] US Food and Drug Administration: Best Way to Get Rid of Used Needles and Other Sharps
[16] US Food and Drug Administration: DOs and DON'Ts of Proper Sharps Disposal
[17] Schoenenberger GA, Monnier M. Characterization of a delta-electroencephalogram (-sleep)-inducing peptide. Proc Natl Acad Sci U S A. 1977;74(3):1282-6.
[18] Schoenenberger GA, Maier PF, Tobler HJ et al. The delta EEG (sleep)-inducing peptide (DSIP). XI. Amino-acid analysis, sequence, synthesis and activity of the nonapeptide. Pflugers Arch. 1978;376(2):119-29.
[19] Kovalzon VM, Strekalova TV. Delta sleep-inducing peptide (DSIP): a still unresolved riddle. J Neurochem. 2006;97(2):303-9.
[20] Graf MV, Saegesser B, Schoenenberger GA. Degradation and aggregation of delta sleep-inducing peptide (DSIP) and two analogs in plasma and serum. Peptides. 1987;8(4):599-603.
[21] Bes F, Hofman W, Schuur J et al. Effects of delta sleep-inducing peptide on sleep of chronic insomniac patients. A double-blind study. Neuropsychobiology. 1992;26(4):193-7.
[22] Schneider-Helmert D, Schoenenberger GA. Effects of DSIP in man. Multifunctional psychophysiological properties besides induction of natural sleep. Neuropsychobiology. 1983;9(4):197-206.
[23] Pomfrett CJ, Dolling S, Anders NR et al. Delta sleep-inducing peptide alters bispectral index, the electroencephalogram and heart rate variability when used as an adjunct to isoflurane anaesthesia. Eur J Anaesthesiol. 2009;26(2):128-34.
[24] Bjartell A, Ekman R, Bergquist S et al. Reduction of immunoreactive ACTH in plasma following intravenous injection of delta sleep-inducing peptide in man. Psychoneuroendocrinology. 1989;14(5):347-55.
[25] Chiodera P, Volpi R, Capretti L et al. Different effects of delta-sleep-inducing peptide on arginine-vasopressin and ACTH secretion in normal men. Horm Res. 1994;42(6):267-72.
[26] Späth-Schwalbe E, Schäfer A, Uthgenannt D et al. Delta-sleep-inducing peptide does not affect CRH and meal-induced ACTH and cortisol secretion. Psychoneuroendocrinology. 1995;20(3):231-7.
[27] Giusti M, Carraro A, Porcella E et al. Delta sleep-inducing peptide administration does not influence growth hormone and prolactin secretion in normal women. Psychoneuroendocrinology. 1993;18(1):79-84.
[28] García-Borreguero D, Wehr TA, Larrosa O et al. Glucocorticoid replacement is permissive for rapid eye movement sleep and sleep consolidation in patients with adrenal insufficiency. J Clin Endocrinol Metab. 2000;85(11):4201-6.